P-82: Investigation of Relationship between Acrosomal Reaction Rate and Sperm DNA Quality in The Fertilization Rate of IVF Undergoing Men

Background: It is estimated that around 15% of the couple at reproductive age are infertile couples, and approximately the half of infertilities are caused by male factor. Defective spermatogenesis is the result of several different disorders, such as endocrinological disorders, disease, malnutrition, genetic defects and maybe environmental condition. Materials and Methods: In this study 60 IVF cycles were investigated in total. In couples undergoing IVF treatment, the sperm and blood were collected. Sperm samples were collected after 2-7 days abstinence. For study of acrosomal reaction (AR), the sperm samples were washed whit Hamsf10 culture media and after incubation in 37°C incubator for 2 hours, the samples were divided into 3 tubes. The first one was control, DMSO was added to the second one and follicular fluid was added to the third one. The sample was kept for 18 hours in 37°C incubator with 5% CO2 and 95% humidity. The samples were stained with double staining method after fixation and then percentage of sperms involved in AR recorded. Finally, the AR of 2nd and 3rd was calculated by extracting the AR of control tube from them. For each of ejaculation two smears of sperm was prepared for DNA analysis. Staining done with Acridine orang and viewed bye fluorescent microscope and the percentage of green colored sperm (single strand) and red (double strand) were recorded. Results: There was a correlation between induced acrosome reaction and follicular fluid as well as fertilization rate. The results also showed that the difference in the mean of acrosome reaction in group with rate 50% were significant (p<0.05). Using ROC analysis with cut-off value of 45% for fertilization, a cutoff value of 10.5% for AR was achieved. Correlation coefficient between rate of normal DNA and fertilization were significant (r=0.041 p<0.05). The mean of normal DNA in cases with rate 50% showed a significant with p value of 0.05. Conclusion: The sperms functional test such as acrosomal reaction could help us to know the real cause of infertility as well as to predict the result of IVF more accurately.